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pcdna3 myc asc  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 myc asc
    Pcdna3 Myc Asc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+myc+asc/pcDNA3-Myc-ASC+(Plasmid+%2373952)/pm40835597-168-0-9
    Average 93 stars, based on 15 article reviews
    pcdna3 myc asc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Helicobacter pylori Avoids the Critical Activation of NLRP3 Inflammasome-Mediated Production of Oncogenic Mature IL-1β in Human Immune Cells
    Article Snippet: .. We transfected 200 ng of pmCherry-C1-proIL1B, 200 ng of pEGFP-C2-NLRP3 (a gift from Christian Stehlik, Addgene plasmid # 73955; http://n2t.net/addgene:73955 ; RRID:Addgene_73955) [ ], 20 ng of pcDNA3-Myc-ASC (a gift from Christian Stehlik, Addgene plasmid # 73952; http://n2t.net/addgene:73952 ; RID:Addgene_73952) [ ] and 100 ng of pCI-caspase1 (a gift from Kate Fitzgerald, Addgene plasmid # 41552; http://n2t.net/addgene:41552 ; RRID:Addgene_41552) [ ] using Lipofectamine 3000 (Thermo Fisher Scientific, Dreieich/Germany) following the manufacturer’s protocol. mCherry-pro-IL-1β was constructed by ligating the pro-IL-1β coding sequence from pCellFree_G03 IL1B (a gift from Kirill Alexandrov, Addgene plasmid # 67066; http://n2t.net/addgene:67066 ; RRID: Addgene_67066) [ ] in-frame into a pmCherry-C1 vector backbone. ..

    Plasmid Preparation:

    Article Title: Helicobacter pylori Avoids the Critical Activation of NLRP3 Inflammasome-Mediated Production of Oncogenic Mature IL-1β in Human Immune Cells
    Article Snippet: .. We transfected 200 ng of pmCherry-C1-proIL1B, 200 ng of pEGFP-C2-NLRP3 (a gift from Christian Stehlik, Addgene plasmid # 73955; http://n2t.net/addgene:73955 ; RRID:Addgene_73955) [ ], 20 ng of pcDNA3-Myc-ASC (a gift from Christian Stehlik, Addgene plasmid # 73952; http://n2t.net/addgene:73952 ; RID:Addgene_73952) [ ] and 100 ng of pCI-caspase1 (a gift from Kate Fitzgerald, Addgene plasmid # 41552; http://n2t.net/addgene:41552 ; RRID:Addgene_41552) [ ] using Lipofectamine 3000 (Thermo Fisher Scientific, Dreieich/Germany) following the manufacturer’s protocol. mCherry-pro-IL-1β was constructed by ligating the pro-IL-1β coding sequence from pCellFree_G03 IL1B (a gift from Kirill Alexandrov, Addgene plasmid # 67066; http://n2t.net/addgene:67066 ; RRID: Addgene_67066) [ ] in-frame into a pmCherry-C1 vector backbone. ..

    Article Title: Nitroxoline is a novel inhibitor of NLRP3-dependent pyroptosis
    Article Snippet: Cells were analyzed using BD FACSLyric flow cytometer (BD Biosciences, NJ, USA). .. pcDNA3-Myc-ASC and pEGFP-C2-NLRP3 were a gift from Christian Stehlik (Addgene plasmid # 73952 ; http://n2t.net/addgene:73952 ; RRID:Addgene_73952; Addgene plasmid # 73955 ; http://n2t.net/addgene:73955 ; RRID:Addgene_73955) [ ]. ..

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation.
    Article Snippet: N/A Oligonucleotides QPCR primers This paper Table S1 sgRNA sequences This paper Table S2 Recombinant DNA Flag-NLRP3 Shi et al.65 pcDNA3-N-Flag-NLRP3, Addgene plasmid # 75127. pCDH vector System Biosciences pCDH-CMV-MCS-EF1-Puro, Addgene plasmid # 2082. psPAX2 vector Trono Lab Packaging and Envelope Plasmids (unpublished) psPAX2, Addgene plasmid # 12260. pMD2.G vector Trono Lab Packaging and Envelope Plasmids (unpublished) pMD2.G, Addgene plasmid # 12259. .. Myc-ASC Bryan et al.66 pcDNA3-Myc-ASC, Addgene plasmid #73952. .. NLRP3-GFP Khare et al.67 pEGFP-C2-NLRP3, Addgene plasmid # 73955. pcDNA3.1(+)-C-HA-ASC GenScript Clone ID: OHu19678C Flag-NLRP3-CS Constructs This paper N/A NLRP3-GFP-CS Constructs This paper N/A pCDH-CMV-MCS-EF1-Puro-Flag-NLRP3 and C126S Constructs This paper N/A ZDHHCs (DHHC1-23) Dr. Masaki Fukata, National Institutes of Natural Sciences of Japan; Zhang et al. N/A pCRISPR-LvSG03-ZDHHC7 sgRNA GeneCopoeia Plasmid #HCP257256-LvSG03-3-B pCRISPR-LvSG03-ZDHHC12 sgRNA GeneCopoeia Plasmid #HCP314389-LvSG03-3-B pLvC9NU-Cas9 GeneCopoeia Plasmid #CP-LvC9NU-01 (Continued on next page) 18 Cell Reports 43, 114070, April 23, 2024

    Article Title: Peli1 facilitates NLRP3 inflammasome activation by mediating ASC ubiquitination
    Article Snippet: pcDNA3-Flag-NLRP3 , Nat Immunol. 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 , Bruce Beutler (Addgene plasmid # 75127). .. pcDNA3-Myc-ASC , Bryan et al. J Immunol. 2009 Mar 1;182(5):3173-82. doi: 10.4049/jimmunol.0802367 . , Christian Stehlik (Addgene plasmid # 73952). .. pcDNA3-Flag-ASC , Nat Immunol. 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 , Bruce Beutler (Addgene plasmid # 75134).

    Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation
    Article Snippet: pMD2.G vector , Trono Lab Packaging and Envelope Plasmids (unpublished) , pMD2.G, Addgene plasmid # 12259.. .. Myc-ASC , Bryan et al. , pcDNA3-Myc-ASC, Addgene plasmid #73952.. .. NLRP3-GFP , Khare et al. , pEGFP-C2-NLRP3, Addgene plasmid # 73955..

    Construct:

    Article Title: Helicobacter pylori Avoids the Critical Activation of NLRP3 Inflammasome-Mediated Production of Oncogenic Mature IL-1β in Human Immune Cells
    Article Snippet: .. We transfected 200 ng of pmCherry-C1-proIL1B, 200 ng of pEGFP-C2-NLRP3 (a gift from Christian Stehlik, Addgene plasmid # 73955; http://n2t.net/addgene:73955 ; RRID:Addgene_73955) [ ], 20 ng of pcDNA3-Myc-ASC (a gift from Christian Stehlik, Addgene plasmid # 73952; http://n2t.net/addgene:73952 ; RID:Addgene_73952) [ ] and 100 ng of pCI-caspase1 (a gift from Kate Fitzgerald, Addgene plasmid # 41552; http://n2t.net/addgene:41552 ; RRID:Addgene_41552) [ ] using Lipofectamine 3000 (Thermo Fisher Scientific, Dreieich/Germany) following the manufacturer’s protocol. mCherry-pro-IL-1β was constructed by ligating the pro-IL-1β coding sequence from pCellFree_G03 IL1B (a gift from Kirill Alexandrov, Addgene plasmid # 67066; http://n2t.net/addgene:67066 ; RRID: Addgene_67066) [ ] in-frame into a pmCherry-C1 vector backbone. ..

    Sequencing:

    Article Title: Helicobacter pylori Avoids the Critical Activation of NLRP3 Inflammasome-Mediated Production of Oncogenic Mature IL-1β in Human Immune Cells
    Article Snippet: .. We transfected 200 ng of pmCherry-C1-proIL1B, 200 ng of pEGFP-C2-NLRP3 (a gift from Christian Stehlik, Addgene plasmid # 73955; http://n2t.net/addgene:73955 ; RRID:Addgene_73955) [ ], 20 ng of pcDNA3-Myc-ASC (a gift from Christian Stehlik, Addgene plasmid # 73952; http://n2t.net/addgene:73952 ; RID:Addgene_73952) [ ] and 100 ng of pCI-caspase1 (a gift from Kate Fitzgerald, Addgene plasmid # 41552; http://n2t.net/addgene:41552 ; RRID:Addgene_41552) [ ] using Lipofectamine 3000 (Thermo Fisher Scientific, Dreieich/Germany) following the manufacturer’s protocol. mCherry-pro-IL-1β was constructed by ligating the pro-IL-1β coding sequence from pCellFree_G03 IL1B (a gift from Kirill Alexandrov, Addgene plasmid # 67066; http://n2t.net/addgene:67066 ; RRID: Addgene_67066) [ ] in-frame into a pmCherry-C1 vector backbone. ..

    other:

    Article Title: Nitroxoline is a novel inhibitor of NLRP3-dependent pyroptosis.
    Article Snippet: Cells were analyzed using BD FACSLyric flow cytometer (BD Biosciences, NJ, USA).

    Expressing:

    Article Title: Nuclear receptor coactivator 6 is a critical regulator of NLRP3 inflammasome activation and gouty arthritis
    Article Snippet: .. The pcDNA3-FLAG-NLRP3 full-length and NLRP3 domain (PYD, NACHT, and LRR) expression plasmids were provided by Eun-Kyeong Jo (Chungnam National University School of Medicine, South Korea) [ ], and pcDNA3-Myc-ASC was purchased from Addgene (Cambridge, WA, USA). .. NLRP3 mutants at the Walker A and Walker B motifs (K232A, D305A, and E306A) [ ] were generated by Macrogen Incorporated (Seoul, Korea).

    Article Title: Caspase-3 targets pro-interleukin-1β (IL-1β) to restrict inflammation.
    Article Snippet: .. The expression vectors pCMV-pro-IL-1b-Flag (75131), pcDNA3-Caspase3-Myc (11813), pcDNA3-Caspase3 (C163A)-Myc (11814), pCMV-Caspase1-Flag (21142), pcDNA3-Myc-ASC (73952) and pcDNA3-N-Flag-NLRP3 (75127) were obtained from Addgene (Watertown, MA, USA). pCMV-pro-IL-1b (D20A)-Flag and pCMV-pro-IL1b (D26A)-Flag were generated from pCMV-pro-IL-1bFlag with a QuikChange XL Site-Directed Mutagenesis Kit (200 517; Agilent Technology, Santa Clara, CA, USA). ..

    Generated:

    Article Title: Caspase-3 targets pro-interleukin-1β (IL-1β) to restrict inflammation.
    Article Snippet: .. The expression vectors pCMV-pro-IL-1b-Flag (75131), pcDNA3-Caspase3-Myc (11813), pcDNA3-Caspase3 (C163A)-Myc (11814), pCMV-Caspase1-Flag (21142), pcDNA3-Myc-ASC (73952) and pcDNA3-N-Flag-NLRP3 (75127) were obtained from Addgene (Watertown, MA, USA). pCMV-pro-IL-1b (D20A)-Flag and pCMV-pro-IL1b (D26A)-Flag were generated from pCMV-pro-IL-1bFlag with a QuikChange XL Site-Directed Mutagenesis Kit (200 517; Agilent Technology, Santa Clara, CA, USA). ..

    Mutagenesis:

    Article Title: Caspase-3 targets pro-interleukin-1β (IL-1β) to restrict inflammation.
    Article Snippet: .. The expression vectors pCMV-pro-IL-1b-Flag (75131), pcDNA3-Caspase3-Myc (11813), pcDNA3-Caspase3 (C163A)-Myc (11814), pCMV-Caspase1-Flag (21142), pcDNA3-Myc-ASC (73952) and pcDNA3-N-Flag-NLRP3 (75127) were obtained from Addgene (Watertown, MA, USA). pCMV-pro-IL-1b (D20A)-Flag and pCMV-pro-IL1b (D26A)-Flag were generated from pCMV-pro-IL-1bFlag with a QuikChange XL Site-Directed Mutagenesis Kit (200 517; Agilent Technology, Santa Clara, CA, USA). ..



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